PK ELISA KITS
DeQuanto® Adalimumab (Humira®) PK ELISA Kit
Product Description
“This Adalimumab ELISA kit has been developed for specific quantification of Adalimumab concentration in human serum or plasma with high sensitivity and reproducibility.”
Test Principle
The DeQuanto® Adalimumab (Humira®) PK ELISA kit is an ELISA based immunoassay. The Adalimumab present in the sample binds to pre coated TNF-α on polystyrene microtiter plate. Non bound or the excess substances are removed by washing. Horseradish Peroxidase (HRP) labeled antibody is added to the wells which binds to the TNF/Adalimumab complex in the wells. Following a wash to remove any unbound antibody-enzyme reagent, substrate solution is added to the wells. A color product is formed in proportion to the amount of Adalimumab present in the sample or standards. The color development is stopped by addition of a stop solution. The absorbance is measured at 450 nm in a microtiter plate reader. The concentration of Adalimumab in a sample can be interpolated from the standard curve.
| Catalouge No. | PK1002 |
|---|---|
| Pack Size : | 96 well Microtiter Plate |
| Assay Range | 5.2 – 200 ng/ml |
| Assay Format | Indirect |
| Sensitivity | 5.2 ng/ml |
| Accuracy & Precision | Intra-Assay and Inter-Assay: CV<20% |
| Assay Matrix | Human serum and plasma |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |