PK ELISA KITS
DeQuanto® Ranibizumab (Lucentis®) PK ELISA Kit
Product Description
“This Ranibizumab ELISA kit has been developed for specific quantification of Ranibizumab concentration in human serum or plasma with high sensitivity and reproducibility.”
Test Principle
“The DeQuanto® Ranibizumab (Lucentis®) PK ELISA kit is an ELISA based immunoassay. Standards and diluted samples (serum/ plasma) are incubated in the polystyrene microtiter plate pre coated with recombinant Human vascular endothelial growth factor-A (rhVEGF-A). The Ranibizumab present in the samples binds to the coated rhVEGF-A. Nonbound or the excess substances are removed by washing. Horseradish Peroxidase (HRP) labeled antibody is added to the wells which binds to the rhVEGF-A-Ranibizumabcomplex in the wells. Following a wash to remove any unbound antibody-enzyme reagent, substrate solution is added to the wells. A color product is formed in proportion to the amount of Ranibizumab present in the sample or standards. The color development is stopped by addition of a stop solution. The absorbance is measured at 450 nm in a microtiter plate reader. The concentration of Ranibizumab in a sample can be interpolated from the
standard curve.”
| Catalouge No. | PK1006 |
|---|---|
| Pack Size : | 96 well Microtiter Plate |
| Assay Range | 1250 – 9.77 ng/ml |
| Assay Format | Indirect |
| Sensitivity | 4.88ng/ml |
| Accuracy & Precision | Intra-Assay and Inter-Assay: CV<20% |
| Assay Matrix | Human serum and plasma |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |