Cytokines ELISA Kits
DeQuanto® TIGHT JUNCTION PROTEIN-1 (TJP1) DETECTION ELISA KIT
Product Description
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TJP1 in human tissue homogenates, cell lysates and other biological fluids.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to TJP1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TJP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TJP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TJP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Catalouge No. | QT4110 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 0.156-10 ng/ml |
| Assay Format | Sandwich |
| Sensitivity | < 0.059ng/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Human tissue homogenates, cell lysates and other biological fluids. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |