Cytokines ELISA Kits
DeQuanto® Bone Morphogenetic Protein-8 B(BMP8B) ELISA KIT
Product Description
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BMP8B in human serum, plasma, tissue homogenates and other biological fluids.
Test Principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP8B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BMP8B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMP8B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BMP8B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Catalouge No. | QT4109 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 15.6-1,000pg/mL |
| Assay Format | Sandwich |
| Sensitivity | < 7pg/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Human serum, plasma, tissue homogenates and other biological fluids. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |