Cytokines ELISA Kits
DeQuanto® PHYPOXIA INDUCIBLE FACTOR-1-ALPHA (HIF1α) DETECTION ELISA KIT
Product Description
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HIF1a in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to HIF1a. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HIF1a, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HIF1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Catalouge No. | QT4107 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 0.156-10 ng/ml |
| Assay Format | Sandwich |
| Sensitivity | < 0.061ng/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |