Cytokines ELISA Kits
DeQuanto® 2-3-BISOHOSPHOGLYCERATE (2-3-BPG) DETECTION ELISA KIT
Product Description
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of 2,3-BPG in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 2,3-BPG has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 2,3-BPG and unlabeled 2,3-BPG (Standards or samples) with the pre-coated antibody specific to 2,3-BPG. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 2,3-BPG in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 2,3-BPG in the sample.
| Catalouge No. | QT4105 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 1.23-100 µg/ml |
| Assay Format | Sandwich |
| Sensitivity | < 0.53μg/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |