Cytokines ELISA Kits
DeQuanto® ANGIOTENSIN-II DETECTION ELISA KIT (High Sensitive)
Product Description
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to AngII has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled AngII and unlabeled AngII (Standards or samples) with the pre-coated antibody specific to AngII. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of AngII in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of AngII in the sample.
| Catalouge No. | QT4071 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 25-2000 pg/ml |
| Assay Format | Competitive inhibition |
| Sensitivity | < 9.17pg/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Competitive inhibition |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |