Small Molecule ELISA Kit
DeQuanto® PEPTIDOGLYCAN DETECTION ELISA KIT
Product Description
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of peptidoglycan in biological agents.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to peptidoglycan has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled peptidoglycan and unlabeled peptidoglycan (Standards or samples) with the pre-coated antibody specific to peptidoglycan. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of peptidoglycan in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of peptidoglycan in the sample.
| Catalouge No. | QT4062 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 1.23-100 ng/ml |
| ELISA plate format | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Format | Competitive inhibition |
| Sensitivity | < 0.49ng/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Biological agents. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |