Small Molecule ELISA Kit

DeQuanto® CHOLESTEROL DETECTION ELISA KIT

Product Description

The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of cholesterol in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Test Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to cholesterol has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled cholesterol and unlabeled cholesterol (Standards or samples) with the pre-coated antibody specific to cholesterol. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of cholesterol in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of cholesterol in the sample.

Catalouge No. QT4057
Pack Size : 12 x 8 Stripwell Microtiter Plate (96 well plate)
Assay Range 6.17-500µg/ml
Assay Format Competitive inhibition
Sensitivity < 2.48μg/ml
Accuracy & Precision Intra-Assay: CV<10% Inter-Assay: CV<15%
Assay Matrix Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Storage Shipped at 2-8°C. Longterm Storage at -20° C
Regulatory For research use only (RUO)
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