Small Molecule ELISA Kit
DeQuanto® CHOLESTEROL DETECTION ELISA KIT
Product Description
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of cholesterol in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to cholesterol has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled cholesterol and unlabeled cholesterol (Standards or samples) with the pre-coated antibody specific to cholesterol. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of cholesterol in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of cholesterol in the sample.
| Catalouge No. | QT4057 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 6.17-500µg/ml |
| Assay Format | Competitive inhibition |
| Sensitivity | < 2.48μg/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |