Small Molecule ELISA Kit
DeQuanto® VITAMIN B7 DETECTION ELISA KIT
Product Description
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Vitamin B7 (VB7) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids..
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique.BSA conjugated VB7 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between BSA conjugated VB7 and unlabeled VB7 (Standards or samples) with HRP conjugated Streptavidin. After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of VB7 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of VB7 in the sample.
| Catalouge No. | QT4056 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 32-20,000 pg/ml |
| Assay Format | Competitive inhibition |
| Sensitivity | < 13pg/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |