Small Molecule ELISA Kit
DeQuanto® Vitamin D3 (VD3) DETECTION ELISA KIT
Product Description
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Vitamin-D3 (VD3) in serum, plasma and other biological fluids.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to VD3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled VD3 and unlabeled VD3 (Standards or samples) with the pre-coated antibody specific to VD3. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of VD3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of VD3 in the sample.
| Catalouge No. | QT4048 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 2.47-200ng/ml |
| Assay Format | Competitive inhibition |
| Sensitivity | < 0.91ng/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Serum, plasma and other biological fluids. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |