Small molecule ELISA Kit
DeQuanto® VITAMIN D2 (VD2) DETECTION ELISA KIT
Product Description
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Vitamin-D2 (VD2) in serum, plasma and other biological fluids.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to VD2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled VD2 and unlabeled VD2 (Standards or samples) with the pre-coated antibody specific to VD2. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of VD2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of VD2 in the sample.
| Catalouge No. | QT4034 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 1.23-100 ng/ml |
| Assay Format | Sandwich |
| Sensitivity | < 0.50ng/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Serum, plasma and other biological fluids |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |