Cytokines ELISA Kits
DeQuanto® IL4 DETECTION ELISA KIT
Product Description
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL4 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to IL4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Catalouge No. | QT4017 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 15.6-1000 pg/ml |
| ELISA plate format | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Format | Sandwich |
| Sensitivity | < 5.9pg/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Human serum and plasma |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |