Cytokines ELISA Kits
DeQuanto® TRANSFORMING GROWTH FACTOR BETA -1 (TGF-b1) DETECTION ELISA KIT
Product Description
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TGFb1 in human serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to TGFb1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGFb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Catalouge No. | QT4016 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 15.6-1000 pg/ml |
| ELISA plate format | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Format | Sandwich |
| Sensitivity | < 5.7pg/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Human serum and plasma |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |