Cytokines ELISA Kits
DeQuanto® ANTI-TNF-α ANTIBODY DETECTION ELISA KIT
Product Description
The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-TNFa Antibody in human serum, plasma and other biological fluids.
Test Principle
The microtiter plate provided in this kit has been pre-coated with TNFa. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-TNFa will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-TNFa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
| Catalouge No. | QT4005 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 3.12-200ng/ml |
| ELISA plate format | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Format | Indirect |
| Sensitivity | < 1.13ng/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Human serum and plasma |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |