Small molecule ELISA Kit
DeQuanto® SALMON CALCITONIN (SCT) DETECTION ELISA KIT
Product Description
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of SCT in biological agents.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to SCT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled SCT and unlabeled SCT (Standards or samples) with the pre-coated antibody specific to SCT. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of SCT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of SCT in the sample.
| Catalouge No. | QT4039 |
|---|---|
| Pack Size : | 12 x 8 Stripwell Microtiter Plate (96 well plate) |
| Assay Range | 78.1-20,000 pg/ml |
| Assay Format | Competitive inhibition |
| Sensitivity | < 31.4pg/ml |
| Accuracy & Precision | Intra-Assay: CV<10% Inter-Assay: CV<15% |
| Assay Matrix | Biological agents. |
| Storage | Shipped at 2-8°C. Longterm Storage at -20° C |
| Regulatory | For research use only (RUO) |