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DeQuantoTM Etanercept ( Enbrel ) PK ELISA KIT (Catalog # PK1003)

CATALOG # : PK1003

Pack Size : 1 x 96 wells

QTY : 1

PRICE : Enquire

  • Product Specifications

    • Sensitivity : 2.34 ng/ml
    • Very cost effective.
    • Assay Duration : 3- 3.5 Hrs
    • Assay Range : 2.34 – 300 ng/ml
    • Assay Matrix : Tested and Validated for Human serum or plasma
    • Specificity : Specifically to anti-TNF drug
    • Interference : No interference observed for Rituximab (Mabthera) in Assay range
    • Note: "Enbrel®" is a registered trademark of Amgen

    The kit is based on very robust ELISA technique and with very high sensitivity.

  • Product Summary


    Etanercept (Enbrel) is a therapeutic fusion protein specific for Tumor Necrosis Factor-Alpha (TNF-alpha) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Enbrel® is a registered trademark of Amgen. Etanercept specifically binds to TNF alpha and blocks its interaction with cell surface TNF receptors and reduces the inflammation and subsequently improves the patient health.

    Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.

    EMA Bio-analytical Method Validation Guidelines and industry-recommended practices for ligand-binding assays were used for validation of this kit. This Etanercept ELISA kit has been developed for specific quantification of Etanercept concentration in human serum or plasma with high sensitivity and reproducibility.

    Assay Principle

    The DeQuantoTM Etanercept (Enbrel) PK ELISA kit is an ELISA based immunoassay. The Etanercept present in the sample binds to pre coated TNF-? on polystyrene microtiter plate. Non bound or the excess substances are removed by washing. Horseradish Peroxidase (HRP) labeled antibody is added to the wells which binds to the TNF/Etanercept complex in the wells. Following a wash to remove any unbound antibody-enzyme reagent, substrate solution is added to the wells. A color product is formed in proportion to the amount of Etanercept present in the sample or standards. The color development is stopped by addition of a stop solution. The absorbance is measured at 450 nm in a microtiter plate reader. The concentration of Etanercept in a sample can be interpolated from the standard curve.